|
KMID : 0903520040470020187
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2004 Volume.47 No. 2 p.187 ~ p.191
|
|
|
Production of Ascorbic acid-2-glucoside from L-Ascorbic acid with Spinach α-Glucosidase
|
|
Chung Ji-Youn
Song Hee-Sang
Bang Won-Gi
|
|
|
Abstract
|
|
|
|
For the production of 2-O-α-D-glucopyranosyl-L-ascorbic acid (ascorbic acid-2-glucoside, AA-2G) from ascorbic acid, the usability of spinach seed as the source of α-glucosidase having transglucosylation activity was studied. The optimum conditions for the production of AA-2G from ascorbic acid and glucose donor were investigated by using crude extract of Spinachia oleracea L. Woosung, the selected source of enzyme. The production of AA-2G was the highest with 1.053 mM when spinach seeds were grown for 2 days after germination. Maltose was the most effective glucose donor, and the optimum concentration of ascorbic acid and maltose were 175 mM and 225 mM, respectively. The optimum concentration of α-glucosidase was 60 units. The most effective buffer was sodium acetate and its optimum concentration was 175 mM. The optimum pH and temperature were 5.0 and 65oC, respectively. Under the optimum condition, 2.14 mM of AA-2G was produced from ascorbic acid after 50 minutes of reaction.
|
|
|
KEYWORD
|
|
|
L-ascorbic acid, spinach, ascorbic acid-2-glucoside, α-glucosidase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|